![]() ![]() ![]() The sensitivity and specificity of this method relative to the Svanova TGEV/PRCV antibody diagnosis kit were 93.5% and 93.8%, respectively. The operating conditions for the ELISA, an antigen concentration of 15 μg/ml, serum dilution of 1:40, blocking solution of 0.5% fetal bovine serum (FBS), serum sample incubation for 90 min, a concentration of horseradish peroxidase (HRP)-spa of 1:5000, incubated for 60 min, incubation of the substrate at room temperature for 10 min, and a cutoff value for the ELISA OD 450⩾0.35, were carried out using a checkerboard titration method. You should receive a call within an hour. Submit the assignment on the lab D2L dropbox in PDF FORMAT. Enter a phone number that can be dialed directly. Homework is assigned every Tuesday during class, and is due the following Monday at 11:59 PM. An indirect enzyme-linked immunosorbent assay (ELISA) for detecting the TGEV nucleocapsid protein antibody was developed after the reactogenicity of the recombinant protein was demonstrated by Western blot. To request a call: Sign in to with your admin credentials. The recombinant PET-N plasmid, which includes the N gene of the Transmissible gastroenteritis virus ( TGEV), was transformed into Escherichia coli BL21 (DE3), and induced at 37☌ with 1.0 mmol/l IPTG (isopropyl β- d-1-thiogalactopyranoside). ![]()
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